Journal: Oncology Research

Article Title: Overexpression of Aristaless-Like Homeobox-4 Inhibits Proliferation, Invasion, and EMT in Hepatocellular Carcinoma Cells

doi: 10.3727/096504016X14685034103833

Figure Lengend Snippet: Expression of ALX4 is undetectable in HCC tissues and cell lines. (A, B) qRT-PCR and Western blot assays were conducted, and the results indicated downregulation of ALX4 in HCC tissues in comparison with the matched noncancerous liver tissues. (C, D) Results of the qRT-PCR and Western blot assays showed that ALX4 was lowly expressed in three HCC cell lines (Huh7, HepG2, and HCCLM3) but was highly expressed in the normal liver cell line L02. * p < 0.05.

Article Snippet: Human HCC cell lines (Huh7, HepG2, and HCCLM3) and normal liver cell line (L02) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Comparison

Journal: Oncology Research

Article Title: Overexpression of Aristaless-Like Homeobox-4 Inhibits Proliferation, Invasion, and EMT in Hepatocellular Carcinoma Cells

doi: 10.3727/096504016X14685034103833

Figure Lengend Snippet: Overexpression of ALX4 inhibits the proliferation of HCC cells. Expression of ALX4 in Huh7 (A) and HepG2 (B) cells after transfection with pCMV-ALX4 was confirmed by Western blot assays. As shown by the MTT assay, overexpression of ALX4 obviously inhibited the proliferation rate of Huh7 (C) and HepG2 (D) cells transfected with pCMV-ALX4 compared to that of the control group transfected with pCMV-scramble. * p < 0.05.

Article Snippet: Human HCC cell lines (Huh7, HepG2, and HCCLM3) and normal liver cell line (L02) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Over Expression, Expressing, Transfection, Western Blot, MTT Assay, Control

Journal: Oncology Research

Article Title: Overexpression of Aristaless-Like Homeobox-4 Inhibits Proliferation, Invasion, and EMT in Hepatocellular Carcinoma Cells

doi: 10.3727/096504016X14685034103833

Figure Lengend Snippet: Overexpression of ALX4 inhibits the migration and invasion of HCC cells. Migration and invasion of Huh7 (A, B) and HepG2 (C, D) cells overexpressing ALX4 were measured by Transwell assays. The assay results were quantitated by counting the migrated and invaded cells in four randomly selected areas. * p < 0.05.

Article Snippet: Human HCC cell lines (Huh7, HepG2, and HCCLM3) and normal liver cell line (L02) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Over Expression, Migration

Journal: Oncology Research

Article Title: Overexpression of Aristaless-Like Homeobox-4 Inhibits Proliferation, Invasion, and EMT in Hepatocellular Carcinoma Cells

doi: 10.3727/096504016X14685034103833

Figure Lengend Snippet: Overexpression of ALX4 inhibits the EMT process in HCC cells. (A) The expression of EMT-related markers E-cadherin, fibronectin, and N-cadherin in Huh7 cells overexpressing ALX4 was detected by Western blot, with β-actin as an internal control. (B) The relative protein expression levels of E-cadherin, fibronectin, and N-cadherin in the different cell groups were analyzed via Glyko BandScan 5.1 software. * p < 0.05.

Article Snippet: Human HCC cell lines (Huh7, HepG2, and HCCLM3) and normal liver cell line (L02) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Over Expression, Expressing, Western Blot, Control, Software

Journal: Journal of Immunology Research

Article Title: Exosomal miR-452-5p Induce M2 Macrophage Polarization to Accelerate Hepatocellular Carcinoma Progression by Targeting TIMP3

doi: 10.1155/2022/1032106

Figure Lengend Snippet: miR-452-5p inhibition suppressed HCC cell migration and invasion. (a) miR-452-5p expression in HCC cells and normal human epithelial cells. (b) miR-452-5p was successfully inhibited by the miR-inhibitor. (c, d) CCK-8 assay and EdU staining of HCC cells with and without miR-452-5p inhibition. Optical density (OD) was measured at 24, 48, and 72 h after transfection. (e) Apoptosis rate was analyzed by flow cytometry. (f, g) Migration and invasion were detected by Transwell assay. ∗∗ P < 0.01.

Article Snippet: HCC cells (Huh-7, MHCC97-L, HCCLM3, and SNU-182), normal human epithelial cell THLE-3, and THP-1 monocytes were obtained from ATCC.

Techniques: Inhibition, Migration, Expressing, CCK-8 Assay, Staining, Transfection, Flow Cytometry, Transwell Assay

Journal: Journal of Immunology Research

Article Title: Exosomal miR-452-5p Induce M2 Macrophage Polarization to Accelerate Hepatocellular Carcinoma Progression by Targeting TIMP3

doi: 10.1155/2022/1032106

Figure Lengend Snippet: miR-452-5p mainly reside in HCC cells-derived exosomes. (a) miR-452-5p in the culture medium of normal epithelial cells and HCC cells. (b) miR-452-5p were encapsulated protected from RNase. (c, d) TEM and WB validation of purified exosomes from SNU-182 and Huh-7 cells. (e) miR-452-5p in HCC cells treated with GW4869 or purified exosomes are analyzed by qRT-PCR. ∗∗ P < 0.01. Scale bar: 200 nm.

Article Snippet: HCC cells (Huh-7, MHCC97-L, HCCLM3, and SNU-182), normal human epithelial cell THLE-3, and THP-1 monocytes were obtained from ATCC.

Techniques: Derivative Assay, Biomarker Discovery, Purification, Quantitative RT-PCR

Journal: Journal of Immunology Research

Article Title: Exosomal miR-452-5p Induce M2 Macrophage Polarization to Accelerate Hepatocellular Carcinoma Progression by Targeting TIMP3

doi: 10.1155/2022/1032106

Figure Lengend Snippet: HCC cells deserved exosomal miR-452-5p induces M2 polarization of macrophages. (a) PKH26-labelled SNU-182-exo and Huh-7-exo. (b) mRNA expression of macrophage markers after coculturing with HCC cell exosome. (c) mRNA expression of M2 macrophage markers. (d) mRNA expression of M2 macrophage markers in macrophages treated with exosomes from miR-452-5p inhibited or overexpressed HCC cells. ∗∗ P < 0.01. Scale bar: 100 μ m.

Article Snippet: HCC cells (Huh-7, MHCC97-L, HCCLM3, and SNU-182), normal human epithelial cell THLE-3, and THP-1 monocytes were obtained from ATCC.

Techniques: Expressing

Journal: Journal of Immunology Research

Article Title: Exosomal miR-452-5p Induce M2 Macrophage Polarization to Accelerate Hepatocellular Carcinoma Progression by Targeting TIMP3

doi: 10.1155/2022/1032106

Figure Lengend Snippet: HCC cells deserved exosomal miR-452-5p accelerates M2 macrophage polarization to stimulate HCC cell migration, invasion in vitro , and tumorigenesis in vivo . (a) Migration and invasion rates of transfected MHCC97-L cells, M-PBS set up as a negative control. (b) Tumorigenicity of xenograft mice models. (c) Tumor volumes were measured each week for three weeks. (d) Tumor weights were measured after mice were sacrificed. ∗∗ P < 0.01.

Article Snippet: HCC cells (Huh-7, MHCC97-L, HCCLM3, and SNU-182), normal human epithelial cell THLE-3, and THP-1 monocytes were obtained from ATCC.

Techniques: Migration, In Vitro, In Vivo, Transfection, Negative Control

Journal: Mediators of Inflammation

Article Title: miR-559 Inhibits Proliferation, Autophagy, and Angiogenesis of Hepatocellular Carcinoma Cells by Targeting PARD3

doi: 10.1155/2022/3121492

Figure Lengend Snippet: miR-559 was downregulated, and PARD3 was upregulated in HCC cells. (a) The expression level of miR-559 in HCC cells. (b) The mRNA expression of PARD3 in HCC cells. (c) The protein level of PARD3 in HCC cells. The values were obtained from three independent experiments and shown as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with L02 cells.

Article Snippet: The HCC cell lines (SNU-387, Huh-7, HCCLM3, and MHCC-97H cells) and the normal human liver cell line L02 were provided by ATCC (Manassas, VA, USA).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Iberverin exhibits antineoplastic activities against human hepatocellular carcinoma via DNA damage-mediated cell cycle arrest and mitochondrial-related apoptosis

doi: 10.3389/fphar.2023.1326346

Figure Lengend Snippet: Iberverin inhibits the viability and proliferation of HCC cells. (A) The chemical structure formula of iberverin. (B) HCCLM3, HepG2, Huh1, Huh7, Huh7.5.1, SMMC7721 and SNU739 cells were incubated with iberverin for 48 h. The 50% inhibiting concentrations (IC 50 ) were calculated based on the MTT assay ( n = 3). (C) Colony formation assay of Huh7, Huh7.5.1 and SNU739 cells incubated with 10, 20, and 40 μmol/L iberverin or DMSO control for 2 weeks ( n = 3). Data were shown as mean ± SD (standard deviation). ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

Article Snippet: The human HCC cell lines HCCLM3, HepG2, Huh7, Huh7.5.1 and SMMC7721 were purchased from the China Typical Culture Collection Center (CCTCC), SNU739 from the Korean Cell Line Bank (KCLB), and Huh1 from the Japanese Collection of Research Bioresources Cell Bank (JCRB).

Techniques: Incubation, MTT Assay, Colony Assay, Control, Standard Deviation